BackgroundConcomitant use of methotrexate (MTX) improves the clinical efficacy of anti-TNF agents in the treatment of rheumatoid arthritis (RA). We aimed to clarify the cytotoxic effect of MTX on transmembrane TNF (tmTNF)-expressing cells treated with anti-TNF agents. MethodsJurkat T cells stably expressing tmTNF were used for the following experiments. https://foldlyers.shop/product-category/pushbutton/